
READ ME File For 'JV00x fidelity tests A'

Dataset DOI: https://doi.org/10.5258/SOTON/D2547

ReadMe Author: Jacob Valentine, https://orcid.org/0009-0004-7956-733X

This dataset supports the thesis entitled "Using RTHS/SICLOPPS to identify inhibitors of the c-MYC/MAX interaction, and developing next generation sequencing approaches to SICLOPPS"
AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2023

DESCRIPTION OF THE DATA
This dataset consists of a PRISM .pzfx file which requires GraphPad PRISM to open; it shows the TdTomato fluorescence of JV00x strains as compared to a positive and negative control.
The presence of TdTomato fluorescence, from colonies grown in spectinomycin containing media, was taken as confirmation of TdTomato and SpecR gene expression, as a proxy for the integrated cassette DNA fidelity.

This dataset contains:
582nm low gain emission 3pts.pzfx

Date of data collection: 
2018-11-28
